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Vitamin Deficiency Blood Test: Check B12, D, Iron & Folate Levels
Stop guessing which nutrient is low, measure B12, vitamin D, iron, folate, and more from one blood draw.
Affiliate disclosure: we earn a commission when you purchase lab tests through links on this site, at no extra cost to you. Learn more
Panels from $183.96 with the 20% promotion (regular prices $229.95–$1,548.95; promotional pricing at time of writing).
At a glance
A vitamin deficiency blood test measures the nutrients most often behind low energy, brain fog, and run-down feelings, vitamin B12, folate, vitamin D, iron and ferritin, magnesium, and zinc. These panels are built for adults who suspect a specific gap and want lab numbers instead of guesswork before changing diet or supplements. You can order online without a doctor's referral and bring the results to your physician for interpretation.
Best suited for
- Adults with ongoing fatigue, low mood, or brain fog who want to check common nutrient gaps with real lab data
- Vegans, vegetarians, and people on restrictive diets where B12, iron, and vitamin D gaps are more likely
- Anyone already supplementing who wants to confirm levels have actually normalized
- People whose doctor suggested keeping an eye on B12, vitamin D, or iron between visits
What's included
B Vitamins Micronutrients Test Panel
Vitamins & Minerals - Advanced
27 Essential Vitamins and Minerals to Spot Deficiencies
Vitamins & Minerals - Comprehensive
Antioxidants Micronutrients Panel
Heavy Metals Micronutrients Blood Test Panel
Key biomarker ranges
Commonly used adult reference ranges for markers this panel measures, labs vary, so always interpret results against your lab's stated ranges and with your doctor.
Commonly used cutoffs: below 20 ng/mL deficient, 20–30 insufficient, 30–60 a frequently cited target band; season and supplementation move levels substantially.
| Zone | Range |
|---|---|
| Deficient | 0–20 ng/mL |
| Insufficient | 20–30 ng/mL |
| Sufficient | 30–60 ng/mL |
| High | 60–100 ng/mL |
Commonly used reference ranges differ by sex, roughly 30–200 ng/mL is typical, with men’s upper bounds higher; inflammation can raise ferritin and mask low iron.
| Zone | Range |
|---|---|
| Low | 0–30 ng/mL |
| Typical range | 30–200 ng/mL |
| Elevated | 200–400 ng/mL |
The importance of testing
When you feel off despite doing the right things, a nutrient gap is one of the simplest possibilities to check, and one of the cheapest to rule in or out with a blood draw. B12, folate, iron, and vitamin D are markers associated with energy metabolism, red blood cell formation, nervous system function, and bone health, and their levels can drift with diet, absorption, medications, and life stage.
Targeted panels make the search efficient. The B Vitamins panel quantifies folate plus vitamins B1, B2, B3, B5, B6, and B12 to surface gaps linked to diet, malabsorption, or increased nutrient needs. The Vitamins & Minerals - Advanced panel covers the classic deficiency workup in one order: ferritin, folate, homocysteine, iron and TIBC, magnesium (serum and RBC), prealbumin, vitamin D with D2 and D3, transferrin, B12, and zinc.
Testing before supplementing matters because more is not automatically better, some nutrients can run high as well as low, and a baseline tells you whether an intervention is actually moving the number. The practical loop is simple: measure, adjust with your doctor's guidance, and retest to confirm the change worked. Any out-of-range value is something to review with your physician, not a medical conclusion on its own.
Part of our complete guide to Nutrient & Vitamin Lab Tests — browse every panel and biomarker guide in this hub.
Promo code
ULTA7EEASave 20% with code ULTA7EEA, current promotion; confirm final pricing on the partner page.
Ready to get your numbers? Order online, visit a nearby draw site, and receive private results to review with your doctor.
Order NowFrequently asked questions
Which blood test checks for vitamin deficiencies?
The Vitamins & Minerals - Advanced panel is the most direct single order: it measures ferritin, folate, homocysteine, iron and TIBC, magnesium (serum and RBC), prealbumin, vitamin D with D2 and D3, transferrin, B12, and zinc. If you want B-vitamin detail, the B Vitamins panel covers folate plus B1, B2, B3, B5, B6, and B12; broader 24- and 27-test panels extend coverage to antioxidants and trace elements.
Do I need a doctor's order to test for vitamin deficiencies?
No, these panels can be ordered directly online through the partner page and collected at a local lab, no doctor's visit required. You receive results in your account and can share anything out of range with your physician.
Can I order this test in New York, New Jersey, or Rhode Island?
Usually not, direct-to-consumer lab testing is restricted or prohibited in New York, New Jersey, and Rhode Island, so residents of those states may be blocked at the partner's checkout. Residents of other US states can generally order online without issue.
Will this test tell me if a deficiency is causing my fatigue?
It will tell you whether your nutrient levels are inside or outside laboratory reference ranges, the tests measure and flag, they are not intended to confirm the cause of symptoms. Low B12, folate, iron, or vitamin D are markers associated with fatigue, so results give you and your doctor concrete data to discuss.
Should I test before starting supplements?
Testing first gives you a baseline, so you know which nutrients actually need attention and can confirm with a retest that levels responded. Some nutrients can run high as well as low, which is another reason to measure rather than guess, and to involve your doctor in dosing decisions.
References
- [1] Vitamin D - Health Professional Fact Sheet — NIH Office of Dietary Supplements
- [2] Evaluation, Treatment, and Prevention of Vitamin D Deficiency: An Endocrine Society Clinical Practice Guideline — Endocrine Society (Journal of Clinical Endocrinology & Metabolism)
- [3] Vitamin D - Health Professional Fact Sheet — NIH Office of Dietary Supplements
- [4] Iron-Deficiency Anemia — StatPearls, NCBI Bookshelf (NIH)
- [5] Limitations of Serum Ferritin in Diagnosing Iron Deficiency in Inflammatory Conditions — International Journal of Chronic Diseases
- [6] Diagnosis of Hemochromatosis — NIDDK (NIH)
- [7] Dose–response relationship of serum ferritin and dietary iron intake with metabolic syndrome and non-alcoholic fatty liver disease incidence: a systematic review and meta-analysis — Frontiers in Nutrition
Reviewed by

Jason August
Founder & Editorial Lead
Jason August is the founder of Metabolic Markers and its parent company, Advancing Development. He has spent his career building and scaling real-world ventures, including as a founding team member at Build-A-Bear Workshop during its growth from startup to global public company, and since then across capital, real estate, adaptive reuse, and hospitality. He founded Metabolic Markers to make blood-biomarker testing easier to understand and access, and oversees the site’s content and editorial accuracy. Jason is not a medical professional; all content on this site is educational, reviewed for accuracy, and never a substitute for advice from a licensed physician.
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